High‐Throughput Parallel Optofluidic 3D‐Imaging Flow Cytometry

3D light‐sheet microscopy is a powerful tool to obtain content‐rich information of cell populations. However, the limited frame rate high‐quantum‐efficiency cameras hinders its application in high‐throughput flow cytometry. Herein, 3D‐imaging cytometry technique based on that can screen thousands cells per second introduced. This method enables fast, parallel optofluidic scanning by performing 1D acoustofluidic focusing multiple under wide‐field with single objective lens. Multicolor cell‐line samples at record detection throughput over 2000 s −1 demonstrated. Furthermore, an unprecedentedly large‐scale 3D‐morphology‐based cytometric analysis order 10 5 Results show system able capture subcellular structures even high throughputs and cellular overlooked 2D‐imaging